Journal: Scientific Reports

Article Title: Biologically active constituents of the secretome of human W8B2 + cardiac stem cells

doi: 10.1038/s41598-018-19855-4

Figure Lengend Snippet: Biological activity profile of W8B2 + CSC-conditioned medium fractionated by ion exchange chromatography. The effect of conditioned medium on survival of ( A ) neonatal rat cardiomyocytes and ( B ) human cardiac microvascular endothelial cells. ( C ) Proliferation of neonatal rat cardiomyocytes represented by percentage of Ki67 + cells. ( D ) Pro-angiogenic tube formation of human cardiac microvascular endothelial cells, assessed by the number of loops formed in vitro . Serum-free medium and serum-free medium supplemented with 5% FCS (FCS) were served as Control and as a positive control (FCS), respectively. Data are shown as mean ± SEM from 4–6 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001 versus Control by one-way ANOVA. Control, serum-free medium; Full, unfractionated conditioned medium; FCS, serum-free medium supplemented with 5% FCS.

Article Snippet: Human cardiac microvascular endothelial cells (HCMECs, Lonza) were plated at 1.5 × 10 4 cells per well of a 48-well plate and cultured in EGM2-MV medium (Lonza).

Techniques: Activity Assay, Ion Exchange Chromatography, In Vitro, Control, Positive Control

Journal: Scientific Reports

Article Title: Biologically active constituents of the secretome of human W8B2 + cardiac stem cells

doi: 10.1038/s41598-018-19855-4

Figure Lengend Snippet: Molecular and biological regenerative properties of extracellular vesicle fractions of W8B2 + CSC-conditioned medium. ( A ) Particle size distribution shows extracellular vesicles with an average modal size of 201 ± 12 nm. ( B ) Electron micrograph images of extracellular vesicles shows near-spherical shape of double membraned vesicles. ( C ) DELFIA protein analysis shows the expression of CD81 in the extracellular vesicles isolated from 2 different biological samples. The effect of extracellular vesicles on survival of ( D ) neonatal rat cardiomyocytes and ( E ) human cardiac microvascular endothelial cells in culture after simulated ischaemia. The effect of extracellular vesicles on ( F ) proliferation of neonatal rat cardiomyocytes proliferation and ( G ) pro-angiogenic tube formation of human cardiac microvascular endothelial cells, assessed by the number of loops formed. Data are shown as mean ± SEM from 4–6 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 versus PBS by Student’s t test. PBS, phosphate-buffered saline; EV, extracellular vesicles.

Article Snippet: Human cardiac microvascular endothelial cells (HCMECs, Lonza) were plated at 1.5 × 10 4 cells per well of a 48-well plate and cultured in EGM2-MV medium (Lonza).

Techniques: Expressing, Isolation, Saline